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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
24/02/2015 |
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Data da última atualização: |
23/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BRAGA, R. M.; SANTANA, M. F.; COSTA, R. V. da; BROMMONSCHENKEL, S. H.; ARAÚJO, E. F. de; QUEIROZ, M. V. de. |
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Afiliação: |
RODRIGO VERAS DA COSTA, CNPMS. |
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Título: |
Transposable elements belonging to the Tc1-Mariner superfamily are heavily mutated in Colletotrichum graminicola. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Mycologia, New York, v. 106, n. 4, p. 629-641, 2014. |
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DOI: |
10.3852/13?262 |
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Idioma: |
Inglês |
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Conteúdo: |
Transposable elements are ubiquitous and constitute an important source of genetic variation in addition to generating deleterious mutations. Several filamentous fungi are able to defend against transposable elements using RIP(repeat-induced point mutation)-like mechanisms, which induce mutations in duplicated sequences. The sequenced Colletotrichum graminicola genome and the availability of transposable element databases provide an efficient approach for identifying and characterizing transposable elements in this fungus, which was the subject of this study. We identified 132 full-sized Tc1-Mariner transposable elements in the sequenced C. graminicola genome, which were divided into six families. Several putative transposases that have been found in these elements have conserved DDE motifs, but all are interrupted by stop codons. An in silico analysis showed evidence for RIP-generated mutations. The TCg1 element, which was cloned from the Brazilian 2908 m isolate, has a putative transposase sequence with three characteristic conserved motifs. However, this sequence is interrupted by five stop codons. Genomic DNA from various isolates was analyzed by hybridization with an internal region of TCg1. All of the isolates featured transposable elements that were similar to TCg1, and several hybridization profiles were identified. C. graminicola has many Tc1-Mariner transposable elements that have been degenerated by characteristic RIP mutations. It is unlikely that any of the characterized elements are autonomous in the sequenced isolate. The possible existence of active copies in field isolates from Brazil was shown. The TCg1 element is present in severalC. graminicola isolates and is a potentially useful molecular marker for population studies of this phytopathogen. Key words: RIP, transposase, transposon MenosTransposable elements are ubiquitous and constitute an important source of genetic variation in addition to generating deleterious mutations. Several filamentous fungi are able to defend against transposable elements using RIP(repeat-induced point mutation)-like mechanisms, which induce mutations in duplicated sequences. The sequenced Colletotrichum graminicola genome and the availability of transposable element databases provide an efficient approach for identifying and characterizing transposable elements in this fungus, which was the subject of this study. We identified 132 full-sized Tc1-Mariner transposable elements in the sequenced C. graminicola genome, which were divided into six families. Several putative transposases that have been found in these elements have conserved DDE motifs, but all are interrupted by stop codons. An in silico analysis showed evidence for RIP-generated mutations. The TCg1 element, which was cloned from the Brazilian 2908 m isolate, has a putative transposase sequence with three characteristic conserved motifs. However, this sequence is interrupted by five stop codons. Genomic DNA from various isolates was analyzed by hybridization with an internal region of TCg1. All of the isolates featured transposable elements that were similar to TCg1, and several hybridization profiles were identified. C. graminicola has many Tc1-Mariner transposable elements that have been degenerated by characteristic RIP mutations. It is unlikely that any of the char... Mostrar Tudo |
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Thesagro: |
Antracnose. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02476naa a2200205 a 4500
001 2009650
005 2017-05-23
008 2014 bl uuuu u00u1 u #d
024 7 $a10.3852/13?262$2DOI
100 1 $aBRAGA, R. M.
245 $aTransposable elements belonging to the Tc1-Mariner superfamily are heavily mutated in Colletotrichum graminicola.$h[electronic resource]
260 $c2014
520 $aTransposable elements are ubiquitous and constitute an important source of genetic variation in addition to generating deleterious mutations. Several filamentous fungi are able to defend against transposable elements using RIP(repeat-induced point mutation)-like mechanisms, which induce mutations in duplicated sequences. The sequenced Colletotrichum graminicola genome and the availability of transposable element databases provide an efficient approach for identifying and characterizing transposable elements in this fungus, which was the subject of this study. We identified 132 full-sized Tc1-Mariner transposable elements in the sequenced C. graminicola genome, which were divided into six families. Several putative transposases that have been found in these elements have conserved DDE motifs, but all are interrupted by stop codons. An in silico analysis showed evidence for RIP-generated mutations. The TCg1 element, which was cloned from the Brazilian 2908 m isolate, has a putative transposase sequence with three characteristic conserved motifs. However, this sequence is interrupted by five stop codons. Genomic DNA from various isolates was analyzed by hybridization with an internal region of TCg1. All of the isolates featured transposable elements that were similar to TCg1, and several hybridization profiles were identified. C. graminicola has many Tc1-Mariner transposable elements that have been degenerated by characteristic RIP mutations. It is unlikely that any of the characterized elements are autonomous in the sequenced isolate. The possible existence of active copies in field isolates from Brazil was shown. The TCg1 element is present in severalC. graminicola isolates and is a potentially useful molecular marker for population studies of this phytopathogen. Key words: RIP, transposase, transposon
650 $aAntracnose
700 1 $aSANTANA, M. F.
700 1 $aCOSTA, R. V. da
700 1 $aBROMMONSCHENKEL, S. H.
700 1 $aARAÚJO, E. F. de
700 1 $aQUEIROZ, M. V. de
773 $tMycologia, New York$gv. 106, n. 4, p. 629-641, 2014.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca |
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
04/12/2014 |
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Data da última atualização: |
04/12/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
KESSLER, J. D.; OSÓRIO, M. T. M.; NÖRNBERG, J. L.; OSÓRIO, J. C. S.; ESTEVE, R. M. G.; SOUZA, A. P. B.; ARNONI, R. K.; BORBA, M. F. S.; OLIVEIRA, R. M.; FERREIRA, O. G. L. |
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Afiliação: |
J. D. KESSLER, UFPEL; M. T. M. OSÓRIO, UFGD; J. L. NÖRNBERG, UFSM; J. C. S. OSÓRIO, UFGD; R. M. G. ESTEVE, UFPEL; A. P. B. SOUZA, UFSM; R. K. ARNONI, UFPEL; MARCOS FLAVIO SILVA BORBA, CPPSUL; R. M. OLIVEIRA, UFPEL; O. G. L. FERREIRA, UFPEL. |
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Título: |
Composição química da carne de cabritos abatidos em idades diferentes. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Archivos de Zootecnia, Córdoba, v. 63, n. 241, p. 153-160, enero-marzo, 2014. |
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Idioma: |
Português |
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Thesagro: |
Ovino. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/113023/1/ComposicaoKessler.pdf
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Marc: |
LEADER 00699naa a2200229 a 4500
001 2001608
005 2014-12-04
008 2014 bl uuuu u00u1 u #d
100 1 $aKESSLER, J. D.
245 $aComposição química da carne de cabritos abatidos em idades diferentes.$h[electronic resource]
260 $c2014
650 $aOvino
700 1 $aOSÓRIO, M. T. M.
700 1 $aNÖRNBERG, J. L.
700 1 $aOSÓRIO, J. C. S.
700 1 $aESTEVE, R. M. G.
700 1 $aSOUZA, A. P. B.
700 1 $aARNONI, R. K.
700 1 $aBORBA, M. F. S.
700 1 $aOLIVEIRA, R. M.
700 1 $aFERREIRA, O. G. L.
773 $tArchivos de Zootecnia, Córdoba$gv. 63, n. 241, p. 153-160, enero-marzo, 2014.
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Embrapa Pecuária Sul (CPPSUL) |
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